Characteristics of a Monolithic Molecularly Imprinted Column and Its Application for Chromatographic Separation

Hongyuan Yan; Kyung Ho Row

Journal of Industrial and Engineering Chemistry, Vol.13, No.4, 552-557, July, 2007

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Characteristics of a Monolithic Molecularly Imprinted Column and Its Application for Chromatographic Separation

Hongyuan Yan, and Kyung Ho Row^†

Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, Incheon,402-751, Korea

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Monolithic molecularly imprinted columns were designed and prepared by in situ thermal-initiated copolymerization for rapid separation of the xanthine derivatives such as caffeine, theobromine, and theophylline. Molecular recognition was found to be dependent on the structures and arrangements of functional groups of the imprinted molecule and the cavities of the MIP. The morphological characteristics of the monolithic MIP were investigated using scanning electron microscopy, which showed that both mesopores and macropores were formed in the imprinted monolith. The effects of the chromatographic separation conditions on the molecular recognition were investigated; hydrogen bonding and hydrophobic interactions played an important role in the retention and separation. Thermodynamic data (△△H and △△S) obtained from Van’t Hoff plots revealed an enthalpy-controlled separation. The present method is very simple compared with the bulk MIP procedure; the macroporous structure has excellent separation properties.

Keywords:monolithic column;molecularly imprinted polymer;in situ polymerization;xanthine derivatives

[References]

Whitcombe MJ, Martin L, Vulfson EN, Chromatographia, 47, 457 (1998)
Hong JM, J. Ind. Eng. Chem., 4(3), 226 (1998)
Yan HY, Row KH, Int. J. Mol. Sci., 7, 155 (2006)
Peter S, Schweitz L, Nilsson S, Electrophoresis, 24, 3892 (2003)
Wei HS, Tsai YL, Wu JY, Chen H, J. Chromatogr. B, 836, 57 (2006)
Lavignac N, Allender CJ, Brain KR, Anal. Chim. Acta, 510, 139 (2004)
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Xie J, Zhu L, Luo H, Zhou L, Li C, Xu X, J. Chromatogr. A, 934, 1 (2001)
Huang XD, Zou HF, Chen XM, Luo QZ, Kong L, J. Chromatogr. A, 984, 273 (2003)
Yin J, Yang G, Chen Y, J. Chromatogr. A, 1090, 68 (2005)
Ye L, Mosbach K, React. Funct. Polym., 48, 149 (2001)
Dickert FL, Lieberzeit P, Tortschanoff M, Sens. Actuators B-Chem., 65, 186 (2000)
Kriz O, Ramstrom O, Mosbach K, Anal. Chem., 69, 345A (1997)
Hirayama K, Sakai Y, Kameoka K, Noda K, Naganawa R, Sens. Actuators B-Chem., 86, 20 (2002)
Yoshikawa M, Fujisawa T, Izumi J, Kitao T, Sakamoto S, Anal. Chim. Acta, 365, 59 (1998)
Bereczki A, Tolokan A, Horvai G, Horvath V, Lanza F, Hall AJ, Sellergren B, J. Chromatogr. A, 930, 31 (2001)
Martin PD, Jones GR, Stringer F, Wilson ID, Analyst, 128, 345 (2003)
Jin Y, Row KH, J. Ind. Eng. Chem., 12(3), 494 (2006)
Zhang L, Cheng G, Fu C, React. Funct. Polym., 56, 167 (2003)
Haginaka J, Kagawa C, J. Chromatogr. A, 948, 77 (2002)
Li WH, Stover HDH, Macromolecules, 33(12), 4354 (2000)
Sellergren B, Ruckert B, Hall AJ, Adv. Mater., 14, 1204 (2002)
Liu HY, Row KH, Yang GL, Chromatographia, 61, 429 (2005)
Li H, Liu Y, Zhang Z, Liao H, Nie L, Yao S, J. Chromatogr. A, 1098, 66 (2005)
Huang XD, Qin F, Chen XM, Liu YQ, Zou HF, J. Chromatogr. B, 804, 13 (2004)
Yan HY, Jin L, Row KH, J. Liq. Chromatogr. Relat. Technol., 28, 3147 (2005)
Wang DX, Hong SP, Row KH, Bull. Korean Chem. Soc., 25, 357 (2004)
Kim H, Guiochon G, Anal. Chem., 77, 1708 (2005)

[Related Articles]

[1] Whitcombe MJ, Martin L, Vulfson EN, Chromatographia, 47, 457 (1998)

[2] Hong JM, J. Ind. Eng. Chem., 4(3), 226 (1998)

[3] Yan HY, Row KH, Int. J. Mol. Sci., 7, 155 (2006)

[4] Peter S, Schweitz L, Nilsson S, Electrophoresis, 24, 3892 (2003)

[5] Wei HS, Tsai YL, Wu JY, Chen H, J. Chromatogr. B, 836, 57 (2006)

[6] Lavignac N, Allender CJ, Brain KR, Anal. Chim. Acta, 510, 139 (2004)

[7] Vlatakis G, Andersson LI, Miller R, Mosbach K, Nature, 361, 645 (1993)

[8] Xie J, Zhu L, Luo H, Zhou L, Li C, Xu X, J. Chromatogr. A, 934, 1 (2001)

[9] Huang XD, Zou HF, Chen XM, Luo QZ, Kong L, J. Chromatogr. A, 984, 273 (2003)

[10] Yin J, Yang G, Chen Y, J. Chromatogr. A, 1090, 68 (2005)

[11] Ye L, Mosbach K, React. Funct. Polym., 48, 149 (2001)

[12] Dickert FL, Lieberzeit P, Tortschanoff M, Sens. Actuators B-Chem., 65, 186 (2000)

[13] Kriz O, Ramstrom O, Mosbach K, Anal. Chem., 69, 345A (1997)

[14] Hirayama K, Sakai Y, Kameoka K, Noda K, Naganawa R, Sens. Actuators B-Chem., 86, 20 (2002)

[15] Yoshikawa M, Fujisawa T, Izumi J, Kitao T, Sakamoto S, Anal. Chim. Acta, 365, 59 (1998)

[16] Bereczki A, Tolokan A, Horvai G, Horvath V, Lanza F, Hall AJ, Sellergren B, J. Chromatogr. A, 930, 31 (2001)

[17] Martin PD, Jones GR, Stringer F, Wilson ID, Analyst, 128, 345 (2003)

[18] Jin Y, Row KH, J. Ind. Eng. Chem., 12(3), 494 (2006)

[19] Zhang L, Cheng G, Fu C, React. Funct. Polym., 56, 167 (2003)

[20] Haginaka J, Kagawa C, J. Chromatogr. A, 948, 77 (2002)

[21] Li WH, Stover HDH, Macromolecules, 33(12), 4354 (2000)

[22] Sellergren B, Ruckert B, Hall AJ, Adv. Mater., 14, 1204 (2002)

[23] Liu HY, Row KH, Yang GL, Chromatographia, 61, 429 (2005)

[24] Li H, Liu Y, Zhang Z, Liao H, Nie L, Yao S, J. Chromatogr. A, 1098, 66 (2005)

[25] Huang XD, Qin F, Chen XM, Liu YQ, Zou HF, J. Chromatogr. B, 804, 13 (2004)

[26] Yan HY, Jin L, Row KH, J. Liq. Chromatogr. Relat. Technol., 28, 3147 (2005)

[27] Wang DX, Hong SP, Row KH, Bull. Korean Chem. Soc., 25, 357 (2004)

[28] Kim H, Guiochon G, Anal. Chem., 77, 1708 (2005)