Poly(vinyl pyrrolidone) Conjugated Lipid System for the Hydrophobic Drug Delivery

Hye Yun Lee; Seol A Yu; Kwan Ho Jeong; Young Jin Kim

Macromolecular Research, Vol.15, No.6, 547-552, October, 2007

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Poly(vinyl pyrrolidone) Conjugated Lipid System for the Hydrophobic Drug Delivery

Hye Yun Lee, Seol A Yu, Kwan Ho Jeong, and Young Jin Kim^†

Department of Applied Chemistry and Biological Engineering, Chungnam National University, Daejeon 305-764, Korea

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Water soluble polymer, poly(vinyl pyrrolidone) was chosen to conjugate with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(succinyl) (N-succinyl DPPE) to make a new drug delivery system. PVP with an amine group (amino-PVP) was polymerized by free radical polymerization. The amine group of amino-PVP was conjugated with the carboxylic group of N-succinyl DPPE. The resultant conjugate could form nanoparticles in the aqueous solution; these nanoparticles were termed a lipid-polymer system. The critical aggregation concentration was measured with pyrene to give a value of 1 × 10-3 g/L. The particle size of the lipid-polymer system, as measured by DLS, AFM and TEM, was about 70 nm. Lipophilic component in the inner part of the lipid-polymer system could derive the physical interaction with hydrophobic drugs. Griseofulvin was used as a model drug in this study. The loading efficiency and release profile of the drug were measured by HPLC. The loading efficiency was about 54%. The release behavior was sustained for a prolonged time of 12 days. The proposed lipid-polymer system with biodegradable and biocompatible properties has promising potential as a passive-targeting drug delivery carrier because of its small particle size.

Keywords:PVP;N-succinyl DPPE;lipid nanoparticle;drug delivery

[References]

Trubetskoy VS, Torchilin VP, Adv. Drug Deliv. Rev., 16, 331 (1995)
Gao Z, Lukyanov AN, Singhal A, Torchilin VP, Nano Lett., 2, 979 (2002)
Sezgin Z, Yuksel N, Baykara T, Eur. J. Pharm. Biopharm., 64, 261 (2006)
Yokoyama M, Satoh A, Sakurai Y, Okano T, Matsumura Y, Kakizoe T, Kataoka K, J. Control. Release, 55, 2 (1998)
Kim SY, Shin IG, Lee MY, Sung YK, Akaike T, Cho CS, J. Control. Release, 51, 13 (1998)
Wang J, Mongayt D, Torchilin VP, J. Drug Target., 13, 73 (2005)
Kwon GS, Okano T, Adv. Drug Deliv. Rev., 21, 107 (1996)
Hong SW, Kim KH, Huh J, Ahn CH, Jo WH, Macromol. Res., 13(5), 397 (2005)
Vinogradov SV, Bronich TK, Kabanov AV, Bioconjugate Chem., 9, 805 (1998)
Torchilin VP, Eur. J. Pharm. Sci., 11, S81 (2000)
Washington C, Adv. Drug Delv. Rev., 20, 131 (1996)
Torchilin VP, Levchenko TS, Whiteman KR, Yaroslavov AA, Tsatsakis AM, Rizos AK, Michailova EV, Shtilman MI, Biomaterials, 22, 3035 (2001)
Rizos AK, Tsikalas I, Tsatsakis AM, Shtilman MI, J. Non-Cryst. Solids, 352, 5055 (2006)
Jung YP, Son YK, Kim JH, Macromol. Res., 15(1), 82 (2007)
Benahmed A, Ranger M, Leroux JC, Pharm. Res., 18, 323 (2001)
Mu Y, Kamada H, Kodaira H, Sato K, Tsutsumi Y, Maeda M, Kawasaki K, Nomizu M, Yamada Y, Mayumi T, Biochem. Biophys. Res. Commun., 264, 763 (1999)
D’Souza AJM, Schowen RL, Topp EM, J. Control. Release, 94, 91 (2004)
Garrec DL, Gori S, Luo L, Lessard D, Smith DC, Yessine MA, Ranger M, Leroux JC, J. Control. Release, 99, 83 (2004)
Lee RJ, Low PS, Biophys. Acta, 1233, 134 (1995)
Finkelstein E, Amichai B, Grunwald MH, Int. J. Antimicrobial Agents, 6, 189 (1996)

[Referenced By]

[Related Articles]

[1] Trubetskoy VS, Torchilin VP, Adv. Drug Deliv. Rev., 16, 331 (1995)

[2] Gao Z, Lukyanov AN, Singhal A, Torchilin VP, Nano Lett., 2, 979 (2002)

[3] Sezgin Z, Yuksel N, Baykara T, Eur. J. Pharm. Biopharm., 64, 261 (2006)

[4] Yokoyama M, Satoh A, Sakurai Y, Okano T, Matsumura Y, Kakizoe T, Kataoka K, J. Control. Release, 55, 2 (1998)

[5] Kim SY, Shin IG, Lee MY, Sung YK, Akaike T, Cho CS, J. Control. Release, 51, 13 (1998)

[6] Wang J, Mongayt D, Torchilin VP, J. Drug Target., 13, 73 (2005)

[7] Kwon GS, Okano T, Adv. Drug Deliv. Rev., 21, 107 (1996)

[8] Hong SW, Kim KH, Huh J, Ahn CH, Jo WH, Macromol. Res., 13(5), 397 (2005)

[9] Vinogradov SV, Bronich TK, Kabanov AV, Bioconjugate Chem., 9, 805 (1998)

[10] Torchilin VP, Eur. J. Pharm. Sci., 11, S81 (2000)

[11] Washington C, Adv. Drug Delv. Rev., 20, 131 (1996)

[12] Torchilin VP, Levchenko TS, Whiteman KR, Yaroslavov AA, Tsatsakis AM, Rizos AK, Michailova EV, Shtilman MI, Biomaterials, 22, 3035 (2001)

[13] Rizos AK, Tsikalas I, Tsatsakis AM, Shtilman MI, J. Non-Cryst. Solids, 352, 5055 (2006)

[14] Jung YP, Son YK, Kim JH, Macromol. Res., 15(1), 82 (2007)

[15] Benahmed A, Ranger M, Leroux JC, Pharm. Res., 18, 323 (2001)

[16] Mu Y, Kamada H, Kodaira H, Sato K, Tsutsumi Y, Maeda M, Kawasaki K, Nomizu M, Yamada Y, Mayumi T, Biochem. Biophys. Res. Commun., 264, 763 (1999)

[17] D’Souza AJM, Schowen RL, Topp EM, J. Control. Release, 94, 91 (2004)

[18] Garrec DL, Gori S, Luo L, Lessard D, Smith DC, Yessine MA, Ranger M, Leroux JC, J. Control. Release, 99, 83 (2004)

[19] Lee RJ, Low PS, Biophys. Acta, 1233, 134 (1995)

[20] Finkelstein E, Amichai B, Grunwald MH, Int. J. Antimicrobial Agents, 6, 189 (1996)