The novel ferrocene-based compound 2-Fc is synthesized through the reaction of ferrocene carbonate acid and epoxy rosin E-51, and characterized by H-1 NMR. The CV behaviors of 2-Fc in different organic solvents are recorded, and the major influential factors, namely, potential scan rate, concentration of electrolyte, and concentration of 2-Fc, and the mechanism of the electrode process are investigated. The CV behaviors of 2-Fc film on electrode in LiClO4 aqueous and organic solutions and the application of 2-Fc in H2PO4- recognition are also studied.