The energy transfer using 7-amino coumarin dyes as donor and rhodamine 590 (Rh6G) as acceptor were investigated in methanol and acetonitrile solubilized non-aqueous reverse micelles using steady state and time resolved fluorescence spectroscopy. For all donors and acceptor we have obtained higher value of overlap integral (J(lambda)) and higher value of Forster distance (R-0). Although we have got higher value of J(lambda) but the rate constant of energy transfer (k(ET)) in almost all systems are small. Coumarin 153-Rh6G pair is the most efficient donor acceptor pair as reflected by the value of k(ET). The different value of energy transfer rate constant in case of different donors indicate the dependence of energy transfer on the structure of donors. (C) 2004 Elsevier B.V. All rights reserved.