Chirality of S- and R-enantiomer of citronelloxy-cyanobiphenyl (CCB) monolayer on a water surface were investigated by Maxwell displacement current (MDC) and optical second harmonic generation (SHG) measurements. While p-s SHG signal could not reveal the presence of chirality, the polarized angle dependence measurement indicated the ratio between chiral and achiral component of the non-linear optical susceptibility as (X-xyz + X-xzy)/(X-xxz- + X-xzx) approximate to 1/40 and approximate to 1/23 for S- and R-CCB, respectively. It was confirmed that our MDC-SHG system has the ability to detect the chirality in monolayer level. (c) 2005 Elsevier B.V. All rights reserved.