Based on a 3-D graphical representation, Yao et al. [Y.H. Yao, X.Y. Nan, T.M. Wang, Chem. Phys. Lett., 411 (2005) 248] make a comparison for the coding sequences of the first exon of beta-globin gene of 11 different species. However, some results in the Tables 5, 9 and 10 are erroneous, and thus the consequent conclusions are wrong. (c) 2006 Elsevier B.V. All rights reserved.