This letter follows a previous publication from our group [C. Montero, A. Gonzalez Urena, J.O. Caceres, M. Morato, J. Najera, H.J. Loesch, European Physical Journal D 26, (2003) 261] where a significant depletion was observed when a supersonic NO beam seeded in He passed through a resonant RF field. Here the phenomenon has been confirmed using different conditions. It is shown how the observed depletion depends on: (i) the rotational NO quantum numbers: (ii) the strength of both the static and RF electric fields. (c) 2006 Elsevier B.V. All rights reserved.