화학공학소재연구정보센터
Chemical Physics Letters, Vol.433, No.1-3, 234-238, 2006
Analysis of'blinking' or'hopping' single molecule signals with a limited number of transitions
Most single molecule fluorescence signals exhibit two characteristic features: sudden intensity changes ('blinking', 'hopping') and a limited length ('bleaching'). I show that the correlation functions of such 'truncated telegraph signals' for Poisson processes contain linear segments and oscillatory features, deviating strongly from the expected exponential decay. I find that the slope of the initial linear section of the normalized signal autocorrelation is a robust and reliable statistical estimate for the rate of sudden intensity changes in single molecule fluorescence signals with 4 to 100 transitions per trace, significantly outperforming an exponential fit. (c) 2006 Elsevier B.V. All rights reserved.