Chemistry Letters, Vol.31, No.6, 558-559, 2002
Effect of cyclen on the prolongation of M intermediate lifetime in the D96N mutant of bacteriorhodopsin
A new kind of chemical additive-cyclen (1,4,7,10-tetraazacyclododecane) was used in BRD96N-PVA films in an attempt to obtain longer M state lifetime. At the highest cyclen concentration of I : 250, the lifetime of M state could be prolonged to 936s. Comparing the lifetimes of the M state in BRD96N suspension and BRD96N-PVA film with the same cyclen content, it is the strong complexing properties of cyclen towards HI that plays the most important role in this prolongation.