Chemistry Letters, Vol.33, No.3, 300-301, 2004
Monophosphate as eminent ligand to bind Ce(IV)/EDTA complex for site-selective DNA hydrolysis
By the use of oligonuclelotides bearing a terminal monophosphate, 10-base gap was formed in substrate DNA and the monophosphates were placed at the edge of gap. Upon the treatment of these systems with Ce(IV)/EDTA complex, the gap-site was selectively hydrolyzed. The monophosphates remarkably promoted both the selectivity and the efficiency of scission.