Chemistry Letters, Vol.33, No.7, 796-797, 2004
Guaiacol oxidation products in the enzyme-activity assay reaction by horseradish peroxidase catalysis
In the horseradish peroxidase (HRP)-catalyzed oxidation of guaiacol (1), trimeric products, and diastercomers of biphenoquinones have been newly identified. The oxidation product was analyzed by HPLC, liquid chromatography electrospray ionization mass spectrometry (LC-ESIMS), and H-1 NMR spectroscopy.