Uniformly sized molecularly imprinted polymers (MIPs) for bisphenol A (BPA) with immobilized intervals of functional monomers utilizing 4,4'-methylenebisphenol as a pseudo template have been prepared. The MIPs through surface modification for the removal of interference showed significant selectivity for BPA retention and removal performance for interference in actual environmental water samples as the HPLC stationary phase compared to those of ordinary MIPs. These MIPs were employed as pretreatment media of column switching HPLC.