Chemistry Letters, Vol.34, No.1, 36-37, 2005
The intramolecular electron transfer between the type 1 Cu and the type 2 Cu in a mutant of Hyphomicrobium nitrite reductase
The intramolecular electron transfer (ET) between the type 1 Cu and the type 2 Cu in the Cys114Ala mutant of Hyphomicrobium denitrificans nitrite reductase has been observed by pulse radiolysis. At pH 5.4-7.0, the ET rates of C114A having one type 1 Cu and one type 2 Cu per monomer are 10(4)-10(5) times as large as those of the native enzyme containing two type 1 Cu sites and one type 2 Cu site.