The binding ability of di-n-butyl phthalate (DBP) by p-sulfonatocalix[n]arenes {SCAn (n = 4, 6, 8)} and adsorption ability of DBP by chitosan beads modified with SCAn were investigated. SCA6 and SCA8 could include DBP in their hydrophobic cavities in aqueous phase. The amount of DBP adsorbed by chitosan beads modified with SCA6 was approximately 5 times as large as that for unmodified chitosan beads.