We have established a new surface-plasmon-resonance (SPR) based affinity biosensor for highly sensitive and selective detection of dopamine (DA) employing a D-3 dopamine receptor (D-RC) and a home-made DA-bovine serum albumin (DA-BSA) conjugate. The proposed biosensor is simple, reproducible and exhibit excellent sensitivity for DA (detection limit = 85 pg/mL (ppt)) without any significant interference from ascorbic acid (AA), uric acid (UA), and other DA analogues.