High-resolution scanning tunneling microscopy (STM) imaging revealed that cyclohexanethiols (CHTs) on Au(111) form high-density self-assembled monolayers (SAMs) with a (5 x 2 root 3)R35 degrees superlattice at a low solution temperature of -20 degrees C. This packing structure is comparable to a (5 x 2,root 10)R48 degrees superlattice formed at room temperature. From this result, it was found that the two-dimensional structure of CHT SAMs on Au(111) was strongly influenced by the solution temperature, unlike alkanethiol SAMs.