A series of trivalent lanthanide complexes were immobilized on SPR sensory chips and protein separation membranes. They worked as alternatives to transition-metal complexes in detection and separation of histidine-tagged ubiquitin protein. Since the highly coordinated complex between NTA ligand, Eu3+ center, and hexahistidine moiety exhibited the enhanced luminescence, the present system provides a further possibility for use in in-situ optical protein imaging process.