The mixture of beta-sheet peptide-PEG diblock copolymer and beta-sheet peptide spread at air/water interface formed a two-dimensional array of the fibrous objects. The fibrous objects constructed by intermolecular hydrogen bonding of beta-sheet peptides were regulated by compressing of the monolayer. When the spread peptide moieties fully located on the water surface, the distance between the fibers was efficiently modulated by the fraction of PEG segments.