Cyclohexene epoxidation with H2O2 was performed over RuO2/TiO2 under various conditions. The support TiO2 prepared by surfactant-assisted templating sol-gel method is nanocrystalline and has mesoporous structure with narrow monomodal pore size distribution. An activator of 1 mol% RuO2 was loaded onto the synthesized TiO2 by incipient wetness impregnation method. The resultant catalyst was characterized by XRD, N-2 adsorption-desorption and TEM analyses. Reaction conditions in the epoxidation reaction, namely reaction temperature, amount of catalyst and concentration of H2O2 in terms of cyclohexene/H2O2 ratio, were systematically optimized to obtain maximum selectivity to cyclohexene oxide. (c) 2006 Elsevier B.V. All rights reserved.