Here we report a facile method to fabricate hollow TiO2 fibers in micro-scale via faithfully replication of sisal fiber. The length of the as-synthesized fibers could reach several millimeters and consist of a bundle of hollow sub-fibers. The fibers have node, pits, and crevices on their surface, which faithfully sustain the characteristics of the template. The phase transformation and photocatalytic activity of the fibers were also studied.