화학공학소재연구정보센터
Journal of Crystal Growth, Vol.232, No.1-4, 481-488, 2001
Video observation of protein crystal growth in the advanced protein crystallization facility aboard the space shuttle mission STS-95
Crystals of polypeptide with sequence (Pro-Pro-Gly)(10) were grown in the Advanced Protein Crystallization Facility during the US Space Shuttle STS-95 Mission. Well diffracting crystals were obtained by dialysis; they provided the highest resolution X-ray data so far collected for a collagen-like triple helical structure (Berisio et al., Acta Crystallogr. D 56 (2000) 55). During the Mission the crystal growth was monitored by a CCD video camera. The image analysis provided information on spatial distribution of crystals, their movements and their growth. The spatial distribution was non-uniform inside the reactors. Limited motions were observed. The linear growth rate indicates that, in some cases, the growth did not cease in the microgravity environment.