Sugar-substituted alpha-amino acid N-carboxyanhydrides (glycoNCAs), i.e., O-(tetra-O-acetyl-beta-D-glucopyranosyl)-L-serine NCA (2a) and O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-beta-D-glucopyranosyl)-L-serin e NCA (2b), were successfully used for the introduction of a mono(glycopeptide) unit into each terminal primary amino group of a dendrimer. Well-defined dendrimer-based artificial glycoconjugates, O-(beta-D-glucopyranosyl)-L-serine-persubstituted poly(amido amine) (PAMAM) dendrimer (3a) and O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-L-serine-persubstituted PAMAM dendrimer (3b), were synthesized by polymer reaction of PAMAM dendrimer with 2a and 2b, respectively, followed by deacetylation with hydrazine monohydrate.