Fabrication of honeycomb-patterned films from amphiphilic dendronized block copolymer (PEO113-b-PDMA(82)) by 'on-solid surface spreading' and 'on-water spreading' method is reported. Highly ordered honeycomb films with quasi-horizontally paralleled double-layered structure can be fabricated by the on-solid surface spreading method. This work raises the possibility that such structures can be formed in amphiphilic dendronized block copolymers and extends the family of source materials.