Reaction Kinetics and Catalysis Letters, Vol.70, No.2, 287-292, 2000
Hydrogen evolution from L-lactate and L-malate with the combination of lactate dehydrogenase or malate dehydrogenase and hydrogenase from A-eutrophus H16
Hydrogen evolution system from L-lactate and L-malate consisting of lactate dehydrogenase or malate dehydrogenase and hydrogenase from cell free extracts of Alcaligenes eutrophus H16 was established. When the solution containing L-lactate, lactate dehydrogenase, NAD and hydrogenase was incubated at 30 degreesC hydrogen evolution was observed. Similarly, the hydrogen evolution was also observed from the L-malate incubated at 30 degreesC.