The cooperative effect on catalyst deactivation of Pt-based catalysts for dehydrogenation reaction was investigated using the combination of Pt-based/SiO2 and Al2O3. The presence of the effect was confirmed by reaction test, CO adsorption, H-2-TPD and TPO. Considering conversion, active site, H-2 uptake and the nature and amount of coke, interestingly, the physical mixture of Pt-based catalyst supported on SiO2 with 100-120 mesh and Al2O3 60-80 mesh was similar to Pt-based Al2O3. The concept of combination of two catalytic functions, acidity and metallic species, will certainly provide the possibility of better catalyst design.