Understanding the biophysics of fluid dynamics within the context of transcriptional regulation. mediated by nuclear factor (NF)-kappaB, is crucial to developing a consensus oil the molecular basis of fluid mechanics and imbalance. Amiloride, art antikaliuretic-diuretic agent, has recently entered the reahn of NF-kappaB as a key player in regulating the molecular association of fluid dynamics with inflammation and oxidative stress. With the identification of flanking regions encoding the amiloride-sensitive channels that are NF-kappaB-responsive, a new theme emerges which underlies the significance of this association. What is the role of NF-kappaB in regulating fluid mechanics-is it a physiologic or immunologic function? Conversely, amiloride is purported as a major regulator of this transcriptional pathway. It is the mainstream of this survey. therefore, to outline current advances on the biophysics and nature of the interaction existing between amliloride, amiloride-sensitive channels. and NF-kappaB, while searching for potential molecular mechanisms. (C) 2004 Published by Elsevier Inc.