The capacity of chondrocytes to synthesize and remodel the extracellular matrix of the articular cartilage is influenced by mechanical forces applied to joints. Either abnormally high or low loads are detrimental to articular cartilage. Experimental work on animals suggests that immobilization can alter proteoglycan synthesis and result in thinning and softening of the articular cartilage. Little is known of the effects of joint immobility on the pattern of genes expressed by chondrocytes. This study focused on the induction of Mcl-1 gene expression in a rat model of knee joint immobilization by the method of differential display PCR. Increase in Mcl-1 gene expression in chondrocytes induced by joint immobilization was confirmed by RT-PCR, Northern blotting, and immunohistochemistry. Our results indicate that chondrocytes respond to the complete absence of joint motion by expressing Mcl-1 gene. This expression may be part of a defense strategy by chondrocytes to overcome the impending chondrocyte death and cartilage degeneration induced by joint immobility. (c) 2005 Elsevier Inc. All rights reserved.