A correspondence analysis of codon usage in human genes revealed, as expected, that the first axis is strongly correlated with the base composition at Synonymous third codon positions. At one extreme of the second axis were localized genes with a high frequency of NCG and CGN codons. The great majority of these sequences were embedded in CpG islands, while the opposite is true for the genes placed at the other extreme. The two main Conclusions of this paper are: (1) the influence of CpG islands on codon usage, and (2) since the second axis is orthogonal (and therefore independent) of the first, GO-rich genes are not necessarily associated with CpG islands. (c) 2006 Elsevier Inc. All rights reserved.