Biochemical and Biophysical Research Communications, Vol.269, No.1, 76-80, 2000
Assignment of overlapping H-1 NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy
The H-1 NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several hey proton signals were strongly overlapped.