Microhole arrays created by laser photoablation of thin polymer films were used to support an organic gel phase prepared by addition of 1,3:2,4-dibenzylidene sorbitol (DBS) to a solution of o-nitrophenyloctyl ether (NPOE) and an appropriate supporting electrolyte. The preparation and properties of the organic gel phase are described and the NPOE-DBS gel is assessed. The polarized microhole supported organic gel interface is shown to be capable of use as an amperometric detector for ions.