In this paper, based on the approach by combining the "functional domain composition" [K.C. Chou, Y.D. Cai, J. Biol. Chem. 277 (2002) 45765] and the pseudo-amino acid composition [K.C. Chou, Proteins Struct. Funct. Genet. 43 (2001) 246; Correction Proteins Struct. Funct. Genet. 2044 (2001) 2060], the Nearest Neighbour Algorithm (NNA) was developed for predicting the protein subcellular location. Very high success rates were observed, suggesting that such a hybrid approach may become a useful high-throughput tool in the area of bioinformatics and proteomics. (C) 2003 Elsevier Science (USA). All rights reserved.