화학공학소재연구정보센터
Electrophoresis, Vol.22, No.16, 3527-3532, 2001
Dynamics of single-stranded DNA migration in denaturing polyacrylamide slab-gel electrophoresis
We describe an original apparatus for the study of the dynamics of single stranded DNA migration. Four detectors based on laser-induced fluorescence (LIF) are equidistantly placed on one migration lane, allowing repeated measurements of the same DNA band at different positions along migration. This article presents the characteristics and performances of this system and focuses on the data analysis, showing how the multiple detection scheme enables the study of band broadening and band resolution during a migration run. Our results suggest the existence of anomalous (northermal) diffusion of DNA molecules during the electrophoretic process.