We show the potential of nonaqueous capillary electrophoresis (NACE) for analyzing enrofloxacin (ENR), ciprofloxacin (CPR), danofloxacin (DAN), difloxacin (DIF), marbofloxacin (MAR), flumequine (FLU), and oxolinic acid (OXA) in pig kidney samples. We have studied the effects of parameters such as the composition of the organic media, the choice of electrolyte, the pH* of the background electrolyte (BGE), the addition of modifiers, and the reversal of electroosmotic flow. Separation was good with 20 mm ammonium acetate, 0.004% polycation hexadimethrine bromide (HDB), and 4% acetic acid (pH* 5.4) in methanol/acetonitrile (50:50 v/v) medium. We used a quick and simple sample preparation method, hydrochloric acid as an extractant and solid-phase extraction (SPE) with Baker C-18 cartridges as the cleanup step. Recoveries for all quinolones were over 80%.