Electrophoresis, Vol.24, No.1-2, 179-184, 2003
An interface chip connection between capillary electrophoresis and thermal lens microscope
A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 pm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 x 10(-7) absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 x 10(-8) M, which was 100 times lower than that of conventional absorbance detection.
Keywords:capillary electrophoresis;dead volume-free connection;microchip;microfabrication;thermal lens microscope