The combined use of solid-phase microextraction (SPME) and different on-line pre concentration strategies for ultrasensitive capillary electrophoresis-ultraviolet (CE-UV) analysis of five pesticides in a single run is investigated. Normal stacking mode (NSM), field-enhanced sample injection (FESI), and stacking with matrix removal (SWMR) are explored to increase the sensitivity of the CE-UV analysis of a selected group of pesti, cides (cyprodinil, cyromazine, pyrifenox, pirimicarb, and pyrimethanil). It could be observed that reverse polarity-stacking with matrix removal (RP-SWMR) provided the best results in terms of sensitivity (enhancement was up to 272-fold compared with normal injection). The separation buffer consisted of 0.4 mm cetyltrimethylammonium chloride (CTAC), 0.4 m acetic acid at pH 4 containing 5% v/v 2-propanol. This approach was then combined with SPME to determine the pesticides in water, apple, and orange juice. The combination of both preconcentration procedures allowed the determination of these pesticides at concentrations down to 2.5 mug/L in water and 3.1 mug/L in juices (i.e., levels well below the maximum residue limits allowed for these compounds). To our knowledge, this is the first report showing the great possibilities of the combined use of SPME, on-line sample preconcentration, and CE for pesticide analysis.