Pyrrole-titania hybrid films were electropolymerized on platinum electrodes and used for the entrapment of glucose oxidase (GOD) through chronoamperometry. The electrodes deposited in a sodium dodecyl sulfate solution showed higher oxidation current values than those deposited in a phosphate buffer solution. A possible mechanism for this difference was proposed based on the increase in the amount of GOD attachment, originating from the slow diffusion rate of large dodecyl sulfate anions. Hybrid-GOD electrodes revealed enhanced sensitivity with the concentration variation of glucose solution compared to pyrrole-GOD electrodes. (C) 2005 The Electrochemical Society.