Multilayered CoNiFe/Cu nanotubes were electrodeposited in nanoporous membranes under pulsed potential conditions from a single electrolyte. Giant magnetoresistance (GMR) measured with the current applied perpendicular to the plane of the layers was obtained at room temperature. This is the first demonstration of GMR in a nanotubular structure. We observed the value of the GMR at room temperature to be sensitive to the alloy layer deposition potential. (c) 2006 The Electrochemical Society.