In this work we report on the growth of a closely stacked double layer of a self-organized TiO2 nanotubes. For that we first anodize Ti in acidic electrolyte containing hydrofluoric acid to form thin nanotube layers. Afterwards we start a second anodization in a different electrolyte, a glycerol/NH4F mixture. This procedure allows us to grow the second layer directly underneath the first one. From scanning electron microscopy and transmission electron microscopy investigations we revealed that the second growth occurs via the tube bottoms of the first layer. These stacked multilayers generate new possibilities to vertically tailor the properties of the self-organized TiO2 nanotube layers. (c) 2007 The Electrochemical Society.