Induced circular dichroism (CD) spectra of the m-diethynylbenzene macrocycles (S)-2 and (R)-2 that have exoannular chiral side chains are observed in a methanol/chloroform (8:2) solution, indicating the formation of chiral, helical aggregates in solution. Solid films prepared on the surface of quartz substrates by spin-coating solutions of (S)-2 also exhibit CD signals that are remarkably dependent on the solvent used for the spin-coating. The relationship between the CD spectra and the morphology of the solid films observed by atomic force microscopy is discussed.