Effect of essential and nonessential amino acid compositions on the in vitro behavior of human mesenchymal stem cells

Kyung-Min Choi; Hee-Hoon Yoon; Young-Kwon Seo; Kye-Yong Song; Soon-Yong Kwon; Hwa-Sung Lee; Yong Soon Park; Young-Jin Kim; Jung-Keug Park

Korean Journal of Chemical Engineering, Vol.24, No.6, 1058-1063, November, 2007

DOI10.1007/s11814-007-0121-8 Export Citation

Effect of essential and nonessential amino acid compositions on the in vitro behavior of human mesenchymal stem cells

Kyung-Min Choi, Hee-Hoon Yoon, Young-Kwon Seo, Kye-Yong Song¹, Soon-Yong Kwon², Hwa-Sung Lee³, Yong Soon Park³, Young-Jin Kim⁴, and Jung-Keug Park^†

Department of Chemical and Biochemical Engineering, Dongguk University, Seoul 100-715, Korea
¹Department of Pathology, Chung-Ang University, Seoul 156-756, Korea
²Department of Orthopedics, Catholic University, Seoul 150-713, Korea
³Department of Medical Information Engineering, Kwangju Health Collage, Kwangju 506-701, Korea
⁴Biomedical Research Institute, Lifecord. Ltd., Suwon 442-749, Korea

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Mesenchymal stem cells (MSCs) from bone marrow appear to be an attractive tool for use in tissue engineering and cell-based therapies due to their multipotent capacity. The majority of studies on MSCs have been restricted to the roles of growth factors, cytokines, and hormones. Based on previous reports demonstrating the important roles of amino acids, we sought to evaluate the effect of essential amino acids (EAs) and nonessential amino acids (NEAs) on the proliferation and differentiation of MSCs. The results showed that the EA/NEA compositions during culture could significantly modulate MSC proliferation and differentiation and, especially, that EAs served as a potent positive modulator in the proliferation of MSCs without causing a deficit in the differentiation capacity of the cells. These results will be very useful in the production of MSC-based cell therapy products for use in the field of tissue engineering and regenerative medicine.

Keywords:Amino Acid;Proliferation;Differentiation;Mesenchymal Stem Cell;Bone Marrow

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[1] Bruder SP, Jaiswal N, Haynesworth SE, J. Cell Biochem., 64, 278 (1997)

[2] Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich S, Lisberg A, Low WC, Largaespad DA, Verfaillie CM, Nature, 428, 41 (2002)

[3] Grove JE, Bruscia E, Krause DS, Stem Cells, 22, 487 (2004)

[4] Bossolasco P, Corti S, Strazzer S, Borsotti C, DelBo R, Fortunato F, Salani S, Quirici N, Bertolini F, Gobbi A, Deliliers GL, PietroComi G, Soligo D, Experimental Cell Res., 295, 66 (2004)

[5] Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF, Tissue Eng., 4, 415 (1998)

[6] Wagner W, Wein F, Seckinger A, Frankhauser M, Wirkner U, Krause U, Blake J, Schwager C, Eckstein V, Ansorge W, Ho AD, Experimental Hematology, 33, 1402 (2005)

[7] Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR, Science, 284, 143 (1999)

[8] Young RG, Butler DL, Weber W, Caplan AI, Gordon SL, Fink DJ, J. Orthop. Res., 16, 406 (1998)

[9] Denk PO, Knorr M, Curr. Eye. Res., 18, 130 (1999)

[10] Fermor B, Urban J, Murray D, Pocock A, Lim E, Francis M, Cell Biol. Int., 22, 635 (1998)

[11] Iyer VR, Eisen MB, Ross DT, Schuler G, Moore T, Lee JC, Science, 283, 83 (1999)

[12] Martin I, Shastri VP, Padera RF, Yang J, Mackay AJ, Langer R, J. Biomed. Mater. Res., 55, 229 (2001)

[13] Marui T, Niyibizi C, Georgescu HI, Cao M, Kavalkovich KW, Levine RE, J. Orthop. Res., 15, 18 (1997)

[14] Hosokawa A, Takaoka T, Katsuta H, Jpn. J. Exp. Med., 41, 273 (1971)

[15] Eagle H, Science, 122, 501 (1955)

[16] Eagle H, J. Exp. Med., 102, 37 (1955)

[17] Eagle H, J. Biol. Chem., 214, 839 (1955)

[18] Szende B, Tyihak E, Lancet, 1(7546), 824 (1968)

[19] Tyihak E, Szende B, Lapis K, Life Sci., 20, 385 (1977)

[20] Morgan JF, Morton HJ, Parker RC, Nutr. Rev., 8, 181 (1950)

[21] Hanss J, Moore GE, Exp. Cell Res., 34, 243 (1964)

[22] Theresa J, Enrico A, Anil H, Sreedhar K, Surgery, 134, 457 (2003)

[23] Yang L, Zhang B, Toku K, Maeda N, Sakanaka M, Tanaka J, Neurosci. Lett., 295, 97 (2000)

[24] Vjayalakshhmi B, Sesikeran B, Udaykumar P, Kalyanasundaram S, Raghunath M, Free. Radic. Biol. Med., 38, 1614 (2005)

[25] Bavister BD, McKiernan SH, Preimplantation embryo development, New York, Springer-Verlag (1993)

[26] Gwatkin RBL, Haidri AA, Exp. Cell Res., 76, 1 (1973)

[27] Gardner DK, Lane M, Biol. Reprod., 48, 377 (1993)

[28] Zhang X, Armstrong DT, Biol. Reprod., 42, 662 (1990)

[29] Rosenkrans CF, First NL, J. Anim. Sci., 72, 434 (1994)