Polycyclic aromatic hydrocarbons (PAHs) as well as nonylphenol (NP) and octylphenol (OP) are substances of major environmental concern because of their adverse health effects on organisms, including endocrine disrupting activity. Seven PAHs, NP and OP have been included in the priority list of substances regulated by 2000/60/EC Water Framework Directive. The present work concerns the investigation of the occurrence of the seven priority PAHs, NP and OP in surface and coastal waters in Central Greece and the Island of Lesvos. Samples were collected and analyzed seasonally for one year from the four reservoirs that supply water to Athens: Mornos, Marathonas, Yliki and Evinos. Samples were also collected from inland waters of the Lesvos Island (the rivers Mylopotamos and Evergetoulas), and from coastal waters (five sampling points) of the Mytilene area, which is the capital city of the Island of Lesvos. The results have shown that in most cases the water samples that were analyzed are free from PAHs, possibly because of lack of PAHs release sources nearby the particular areas, or due to the adsorption of PAHs on sediments. NP occurred at low levels in several cases, while OP was detected only in one sample.