The purpose of the present study was to estimate Cr(VI) removal through biological mechanisms in biofilm reactors operated in SBR operating mode with recirculation using different filter media types. The choice of the support material is of great importance since chromate reduction is followed by the formation of sediments, which causes obstruction of the flow along the filter depth. In order to overcome this awkwardness, we tested the performance of two different support materials, i.e. plastic media and calcitic gravel, in pilot-scale trickling filters. The feed concentrations of Cr(VI) examined were about 5, 30 and 100 mg/l, while the concentration of the organic carbon was constant at 400 mg/l, in order to avoid carbon limitations in the bulk liquid. Plastic media showed better performance at the different Cr(VI) concentrations examined, compared to the gravel media. The removal rates for the plastic media achieved were 4.23 +/- 0.18, 3.62 +/- 0.1 and 3.3 +/-0.08 g Cr(VI)/d, for feed concentration of Cr(VI) about 5, 30 and 100 mg/l respectively, while for the gravel media the corresponding removal rates were 4.11 +/- 0.09, 3.52 +/- 0.06 and 2.5 +/- 0.07 g Cr(VI)/d.