A novel class of gemini pyridinium surfactants with a four-methylene spacer group was synthesized, and their surface-active properties and interactions with polyacrylamide (PAM) were evaluated by surface tension, fluorescence, and viscosity measurements. A comparison between the gemini pyridiniurn surfactants and their corresponding monomers was also made. The cmc's of gemini pyridinium surfactants are much lower than those of the corresponding monomeric surfactants. The C-20 value is about one order of magnitude lower than that of corresponding monomers, and the longer the hydrophobic chains of the surfactants, the lower the cmc value. Surface tension measurements of the surfactantPAM mixed systems show that the critical aggregation concentration (cac) value is much lower than the cmc value of the surfactant system alone. Viscosity measurements of the surfactant-PAM mixed systems show that the relative viscosity of the surfactant-PAM system decreased with increasing concentration of surfactant. Additionally, fluorescence measurements of the surfactant-PAM mixed system suggest the formation of surfactant- polymer aggregates, and the gemini pyridinium surfactant with longer hydrophobic chains have a stronger interaction with PAM, owing to the stronger hydrophobic interaction.