Bacterial strain VKM B-2445 is characterized by ethylenediaminetetraacetate (EDTA) requirement for cell growth. This strain could not grow on glucose and organic acids as the sole sources of carbon and energy, but it was able to metabolize these substrates added to EDTA medium. EDTA initiated assimilation of glucose, succinate, fumarate, malate, and citrate and supplied nitrogen for the biomass production from these substrates. Utilization of primarily nongrowth substrates by strain VKM B-2445 started when EDTA was exhausted or at least considerably degraded.