The use of full-fuel-cycle analysis as a scientific, economic, and policy tool for the evaluation of alternative sources of transportation energy has become increasingly widespread. However, consistent methods for performance of these types of analyses are only now becoming recognized and utilized. The work presented here provides a case study of full-fuel-cycle analysis methods applied to the evaluation of gasoline in the southeastern region of the United States. Results of the study demonstrate the significance of nonvehicle processes, such as fuel refining, in terms of energy expenditure and emissions production. Unique to this work is the application of the MOBILES mobile emissions model in the full-fuel-cycle analysis. Estimates of direct and indirect greenhouse gas production are also presented and discussed using the full-fuel-cycle analysis method.