Inorganic Chemistry, Vol.46, No.22, 9400-9404, 2007
Labeling of the neuropeptide enkephalin with functionalized Tris (pyrazolyl)borate complexes: Solid-phase synthesis and characterization of p-[Enk-OH]COC(6)H(4)TpPtMe(3) and p-[Enk-OH]COC(6)H(4)Tp(Me)Re(CO)(3)
Bioconjugates of the neuropeptide Enkephalin (Enk = -Tyr-Gly-Gly-Phe-Leu-) labeled with organotransition metal tris-pyrazolylborate (Tp) complexes are reported. p-[Enk-OH]COC(6)H(4)TpPtMe(3) and p-[Enk-0H]COC(6)H(4)Tp(Me)Re(CO)(3) have been synthesized by solid-phase peptide synthesis (SPPS), purified by RP-HPLC, and characterized by ESI-mass spectrometry and H-1 NMR and IR spectroscopies. p-[Enk-OH]COC6H4TpPtMe3 and p-[Enk-OH]COC6H4TpMeRe(CO)3 constitute the first examples of Tp complexes employed in SPPS.