화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Chemical Engineering Research, Vol.46, No.2, 430-435, April, 2008
Export Citation
상황버섯의 균사체로부터 다당류의 추출과 정제
Extraction and Purification of Polysaccharides from Phellinus linteus Mycelia
최근호†
  • 한밭대학교 화학공학과, 305-719 대전시 유성구 덕명동 산16-1
Keun Ho Choi†
  • Department of Chemical Engineering, Hanbat National University, San 16-1, Dukmyung-dong, Yuseong-gu, Daejeon 305-719, Korea
E-mail:
초록
상황버섯(Phellinus linteus) 균사체로부터 얻어지는 다당류를 최대화하기 위하여 투석시간(8, 16, 24, 48 h)을 포함하는 추출조건과 정제조건이 다당류의 양에 미치는 영향에 대해 연구하였다. 추출온도(50, 65, 80, 95 ℃)와 건조 균사체의 무게에 대한 물의 부피의 비(10, 15, 20, 25 ml/g), 추출시간(2, 4, 6, 8 h), 에탄올의 최종 농도(70, 75, 80, 85%) 그리고 묵힘시간(1, 4, 8, 16 h)이 증가할수록 다당류의 양은 증가하였다. 투석시간이 24 h까지 증가할수록 다당류의 양은 감소하였으나 그 이상의 투석시간은 다당류의 양을 거의 변화시키지 못했다. 침전용매로는 3가지 용매(에탄올, 메탄올, 아세톤)를 사용하였는데, 다당류의 양은 아세톤, 에탄올, 메탄올의 순서로 감소하였다. 최적 추출조건은 추출온도가 95 ℃이고, 건조 균사체의 무게에 대한 물의 부피의 비가 25 ml/g이며, 추출시간은 8 h인 조건이었다.
To maximize the obtained polysaccharides from Phellinus linteus mycelia, effects of extraction and purification conditions including dialysis time (8, 16, 24, 48 h) on an amount of the polysaccharides were investigated. As extraction temperature (50~95 ℃), ratio of solvent volume to the dry weight of mycelia (10, 15, 20, 25 ml/g), extraction time(2, 4, 6, 8 h), final concentration of ethanol (70, 75, 80, 85%), and aging time (1, 4, 8, 16 h) increased, an amount of the polysaccharides was increased. An increase in precipitation time up to 24 h increased an amount of the polysaccharides but a further increase in precipitation time after 24 h did not changed largely an amount of the polysaccharides. Three precipitation solvents (ethanol, methanol, acetone) were tested. An amount of the polysaccharides was increased in order to acetone, ethanol, and methanol. On the optimal extraction condition, extraction temperature, ratio of water volume to the dry weight of mycelia, and extraction time were 95 ℃, 25 ml/g, and 8 h, respectively.
Keywords:Phellinus Linteus;Polysacchrides;Water Extraction;Purification
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