Application of spent sulfidic caustics for autotrophic denitrification in a MLE process and their microbial characteristics by fluorescence in situ hybridization

Jeung-Jin Park; So-Ra Park; Dong-Jin Ju; Jeong-Keun An; Im-Gyu Byun; Tae-Joo Park

Korean Journal of Chemical Engineering, Vol.25, No.3, 542-547, May, 2008

DOI10.1007/s11814-008-0091-5 Export Citation

Application of spent sulfidic caustics for autotrophic denitrification in a MLE process and their microbial characteristics by fluorescence in situ hybridization

Jeung-Jin Park, So-Ra Park, Dong-Jin Ju, Jeong-Keun An, Im-Gyu Byun, and Tae-Joo Park^†

Department of Environmental Engineering, Pusan National University, Busan 609-735, Korea

E-mail:

Spent sulfidic caustics (SSCs) produced from petrochemical plants contain a high concentration of hydrogen sulfide and alkalinity, and some organic matter. Most of the SSCs are incinerated with the auxiliary fuel causing secondary pollution problems. The reuse of this waste is becoming increasingly important in terms of economical and environmental viewpoints. To denitrify wastewater with a low COD/N ratio, additional carbon sources are required. Therefore, autotrophic denitrification has received increasing attention. In this research, SSCs were injected as electron donors for sulfur-based autotrophic denitrification in a modified Ludzack-Ettinger (MLE) process. According to the variations in the SSCs dosage, the efficiencies of COD, nitrification and TN removal were evaluated. Heterotrophic denitrification by organic matter and autotrophic denitrification by SSCs were also investigated. As a result, adequate injection of SSCs showed stable autotrophic denitrification. To investigate some of the harmful effects of SSCs, fluorescence in situ hybridization (FISH) for nitrifying bacteria and Thiobacillus denitrificans was performed. Ammoniaoxidizing bacteria (AOB) and Nitrospira genus showed a similar pattern. Excessive injection of SSCs made nitrifying bacteria decrease and nitrification failure occur because of the high pH caused by the SSCs. The distribution of T. denitrificans was relatively uniform as SSCs were injected. This result means that T. denitrificans are available at high pH.

Keywords:Autotrophic Denitrification;Spent Sulfidic Caustics;Fluorescence In Situ Hybridization;Thiobacilus denitrificans;Nitrifying Bacteria

[References]

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Zhang TC, Bishop PL, Wat. Environ. Res., 68, 1107 (1996)
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Narcis N, Rebhun M, Scheindorf C, Water Res., 13, 93 (1979)
Skinde JR, Bhagat SK, J. WPCF, 54, 370 (1982)
Park TJ, Lee KH, Kim DS, Kim CW, Water Sci. Technol., 34, 9 (1996)
Lee KH, Lee JH, Park TJ, Korean J. Chem. Eng., 15(1), 9 (1998)
Batchelor B, Lawrence AW, J. WPCF, 50, 1986 (1978)
Claus G, Kutzner HJ, Appl. Microbiol. Biotechnol., 22, 289 (1985)
Koenig L, Liu H, Water Sci. Technol., 34, 496 (1996)
Zhang TC, Lampe DG, Water Res., 33, 599 (1999)
Oh SE, Yoo YB, Young JC, Kim IS, J. Biotechnol., 92, 1 (2001)
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Flere JM, Zhang TC, J. Environ. Eng., 8, 721 (1999)
Sheu SH, Weng HS, Water Res., 35, 2017 (2001)
Sipma J, Svitelskaya A, van der Mark B, Pol LWH, Lettinga G, Buisman CJN, Janssen AJH, Water Res., 38, 4331 (2004)
Byun IG, Ko JH, Jung YR, Lee TH, Kim CW, Park TJ, Korean J. Chem. Eng., 22(6), 910 (2005)
Schramm A, De Beer D, Wagner M, Amann R, Appl. Environ. Microbiol., 64, 3480 (1998)
Jang A, Bishop PL, Okabe S, Lee SG, Kim IS, Water Sci. Technol., 47, 49 (2002)
Hur SH, Park JJ, Kim YJ, Yu JC, Byun IG, Lee TH, Park TJ, Korean J. Chem. Eng., 24(1), 93 (2007)
Sharma B, Albert RC, Water Res., 11, 897 (1977)
Choi ES, Lee HS, Korean J. Chem. Eng., 13(4), 364 (1996)
Manz W, Wagner M, Amann R, Schleifer KH, Water Res., 28, 1715 (1994)
APHA, Standard methods for the examining of water and wastewater, 20th, American Public Health Association, Washington DC, USA (1998)
Villaverde S, Garcia-Encina PA, Fdz-Polanco F, Water Res., 31, 1180 (1997)
Laia CL, Jesus GG, J. Microbiol. Methods, 57, 69 (2004)
Daims H, Bruhl A, Amann R, Schleifer KH, Wagner M, System Appl. Microbiol., 22, 434 (1999)
Mobarry BK, Wagner M, Urbain V, Rittmann E, Stahl DA, Appl. Environ. Microbiol., 62, 2156 (1996)
Wagner M, Rath G, Koops HP, Flood J, Amann R, Water Sci. Technol., 34(1-2), 237 (1996)
Daims H, Nielsen PH, Nielsen JL, Juretschko S, Wagner M, Water Sci. Technol., 41(4-5), 85 (2000)
Uki Y, Development of mRNA FISH by catalyed reporter deposition (CARD) methods targeting function gene of Aps in sulfate reducing (CARD) methods targeting function gene of Aps in sulfate reducing, M.S.thesis, Department of environmental and urban engineering, Nagaoka national college of technology, Japan (2006)

[Referenced By]

[1] Surmacz-Gorska J, Cichon A, Miksch K, Proceedings of the Env. Biotech., 1, 78 (1996)

[2] Zhang TC, Bishop PL, Wat. Environ. Res., 68, 1107 (1996)

[3] Montieth HD, Bridle TR, Sutton PM, Evaluation of industrial waste carbon sources for biological denitrification, Environ. Canada Wastewater Tech. Centre Report, No. EPS 4-WP-79-9 (1979)

[4] Narcis N, Rebhun M, Scheindorf C, Water Res., 13, 93 (1979)

[5] Skinde JR, Bhagat SK, J. WPCF, 54, 370 (1982)

[6] Park TJ, Lee KH, Kim DS, Kim CW, Water Sci. Technol., 34, 9 (1996)

[7] Lee KH, Lee JH, Park TJ, Korean J. Chem. Eng., 15(1), 9 (1998)

[8] Batchelor B, Lawrence AW, J. WPCF, 50, 1986 (1978)

[9] Claus G, Kutzner HJ, Appl. Microbiol. Biotechnol., 22, 289 (1985)

[10] Koenig L, Liu H, Water Sci. Technol., 34, 496 (1996)

[11] Zhang TC, Lampe DG, Water Res., 33, 599 (1999)

[12] Oh SE, Yoo YB, Young JC, Kim IS, J. Biotechnol., 92, 1 (2001)

[13] van der Hock JP, Hijnen WA, van Bennekom CA, Mijnarends BJ, J. Wat. SRT-Aqua, 41, 209 (1992)

[14] van der Hock JP, Kappelhof JWNM, Hijen WAM, J. Chem. Tech. Biotech., 54, 197 (1992)

[15] van der Hock JP, Kappelhof JWNM, Schippers JC, J. Wat. SRT-Aqua, 43, 84 (1994)

[16] Flere JM, Zhang TC, J. Environ. Eng., 8, 721 (1999)

[17] Sheu SH, Weng HS, Water Res., 35, 2017 (2001)

[18] Sipma J, Svitelskaya A, van der Mark B, Pol LWH, Lettinga G, Buisman CJN, Janssen AJH, Water Res., 38, 4331 (2004)

[19] Byun IG, Ko JH, Jung YR, Lee TH, Kim CW, Park TJ, Korean J. Chem. Eng., 22(6), 910 (2005)

[20] Schramm A, De Beer D, Wagner M, Amann R, Appl. Environ. Microbiol., 64, 3480 (1998)

[21] Jang A, Bishop PL, Okabe S, Lee SG, Kim IS, Water Sci. Technol., 47, 49 (2002)

[22] Hur SH, Park JJ, Kim YJ, Yu JC, Byun IG, Lee TH, Park TJ, Korean J. Chem. Eng., 24(1), 93 (2007)

[23] Sharma B, Albert RC, Water Res., 11, 897 (1977)

[24] Choi ES, Lee HS, Korean J. Chem. Eng., 13(4), 364 (1996)

[25] Manz W, Wagner M, Amann R, Schleifer KH, Water Res., 28, 1715 (1994)

[26] APHA, Standard methods for the examining of water and wastewater, 20th, American Public Health Association, Washington DC, USA (1998)

[27] Villaverde S, Garcia-Encina PA, Fdz-Polanco F, Water Res., 31, 1180 (1997)

[28] Laia CL, Jesus GG, J. Microbiol. Methods, 57, 69 (2004)

[29] Daims H, Bruhl A, Amann R, Schleifer KH, Wagner M, System Appl. Microbiol., 22, 434 (1999)

[30] Mobarry BK, Wagner M, Urbain V, Rittmann E, Stahl DA, Appl. Environ. Microbiol., 62, 2156 (1996)

[31] Wagner M, Rath G, Koops HP, Flood J, Amann R, Water Sci. Technol., 34(1-2), 237 (1996)

[32] Daims H, Nielsen PH, Nielsen JL, Juretschko S, Wagner M, Water Sci. Technol., 41(4-5), 85 (2000)

[33] Uki Y, Development of mRNA FISH by catalyed reporter deposition (CARD) methods targeting function gene of Aps in sulfate reducing (CARD) methods targeting function gene of Aps in sulfate reducing, M.S.thesis, Department of environmental and urban engineering, Nagaoka national college of technology, Japan (2006)