The difference between a continuous stirred-tank reactor (CSTR) and a batchwise reactor has been shown for the enzymatic kinetic resolution of enantiomers. By macroscopic reactor balancing new quantitative relationships between enzyme enantioselectivity, substrate or product enantiomeric excess, and the extent of conversion have been deduced for chiral resolution in a CSTR They were experimentally verified for the esterase-catalyzed resolution of racemic methyl 2-chloropropionate. This reaction was performed both in a batch system and in an enzyme membrane reactor functioning as a CSTR. As expectecd, the batchwise reaction was superior.