Bio-denitrification of the nitrate waste solution from the lagoon sludge in a continuous bio-reduction process

O Mi Lee; Jong Hyeok Oh; Marie Libert; Doo Seong Hwang; Yun Dong Choi; Jin Ho Park; Un Soo Chung; Byung Real Jo; Min Ju Kim; Sang- Joon Lee

Korean Journal of Chemical Engineering, Vol.25, No.4, 787-792, July, 2008

DOI10.1007/s11814-008-0129-8 Export Citation

Bio-denitrification of the nitrate waste solution from the lagoon sludge in a continuous bio-reduction process

O Mi Lee, Jong Hyeok Oh¹, Marie Libert², Doo Seong Hwang, Yun Dong Choi, Jin Ho Park, Un Soo Chung, Byung Real Jo³, Min Ju Kim⁴, and Sang- Joon Lee^{4, †}

Korea Atomic Energy Research Institute, Daejeon 305-600, Korea
¹Korea Research Institute of Chemical Technology, Daejeon 306-600, Korea
²French Atomic Energy Commission, Center de Cadarache, Saint-Paul-Lez-Durance CEDEX, 13108, France
³Department of Environmental Engineering, Chungju National University, Chungbuk 308-702, Korea
⁴Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea

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The treatment of lagoon sludge is a serious task during the decommissioning of a uranium conversion plant. The main component of the sludge is ammonium nitrate, which is a very explosive material. Therefore, biological removal of the ammonium nitrate would be an attractive process. In this work, the bio-denitrification of the nitrate solution from lagoon sludge with a continuous fermentation process was studied. The optimal conditions for removal of nitrate by Pseudomonas halodenitrificans in the continuous bio-reduction process were a C/N ratio and pH of 3.1 and 7.5, respectively, with CO2 gas control, and five times the microelements as used in a batch culture, at 30 oC. It was concluded that bio-denitrification could be applicable to lagoon sludge waste, but with some limitations.

Keywords:Nitrate;Denitrification;Pseudomonas halodenitrificans;Continuous Culture

[References]

Bohler E, Haldenwange L, Schwabe G, Water Sci. Technol., 29(10-11), 497 (1994)
Bockle R, Rohmanm U, Wertz A, Aquacult., 5, 286 (1989)
Cole J, Tibtech., 11, 368 (1993)
Dahal M, Young WL, J. Water Pollut. Control Fed., 60, 1670 (1988)
Chang IS, Park JH, Kim EH, Park JJ, Kim TJ, Jung WM, Jung KC, Choi JH, Development of nuclear fuel: Improvement of reconversion process for nuclear fuel, Report KAERI/RR-1005/90 (1991)
Oh JH, Lee OM, Hwang DS, Choi YD, Hwang ST, Jo BR, Park JH, J. of the Korean Radioactive Waste Society, 4(2), 153 (2006)
Park DH, Park YK, J. Microbiol. Biotechnol., 11(3), 406 (2001)
Dhamole PB, Nair RR, D'Souza SF, Lele SS, Bioresour. Technol., 98(2), 247 (2007)
Paccard E, Besnainou B, Molleta R, Method for biodepolluting effluents with high concentrations of pollutants and strain selection process usable with said method, French patent: WO-97-6107 (ATTACHED FILE) (1995)
Paccard E, Denitrification biologique d’effluents industriels a fortes concentrations en nitrates, Ph thesis (in French)-University of Marseille-Provence (1995)
Kim YJ, Song YC, Kim JO, Park HS, Kor. J. Microbiol. Biotechnol., 33, 65 (2005)

[Referenced By]

[1] Bohler E, Haldenwange L, Schwabe G, Water Sci. Technol., 29(10-11), 497 (1994)

[2] Bockle R, Rohmanm U, Wertz A, Aquacult., 5, 286 (1989)

[3] Cole J, Tibtech., 11, 368 (1993)

[4] Dahal M, Young WL, J. Water Pollut. Control Fed., 60, 1670 (1988)

[5] Chang IS, Park JH, Kim EH, Park JJ, Kim TJ, Jung WM, Jung KC, Choi JH, Development of nuclear fuel: Improvement of reconversion process for nuclear fuel, Report KAERI/RR-1005/90 (1991)

[6] Oh JH, Lee OM, Hwang DS, Choi YD, Hwang ST, Jo BR, Park JH, J. of the Korean Radioactive Waste Society, 4(2), 153 (2006)

[7] Park DH, Park YK, J. Microbiol. Biotechnol., 11(3), 406 (2001)

[8] Dhamole PB, Nair RR, D'Souza SF, Lele SS, Bioresour. Technol., 98(2), 247 (2007)

[9] Paccard E, Besnainou B, Molleta R, Method for biodepolluting effluents with high concentrations of pollutants and strain selection process usable with said method, French patent: WO-97-6107 (ATTACHED FILE) (1995)

[10] Paccard E, Denitrification biologique d’effluents industriels a fortes concentrations en nitrates, Ph thesis (in French)-University of Marseille-Provence (1995)

[11] Kim YJ, Song YC, Kim JO, Park HS, Kor. J. Microbiol. Biotechnol., 33, 65 (2005)